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KMID : 0613820070170040470
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Journal of Life Science
2007 Volume.17 No. 4 p.470 ~ p.475
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Study of Genetic Diversity and Taxonomy of Genus Sorbus in Korea Using Random Amplified Polymorphic DNA
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Park So-Hye
Kim Se-Hyun
Huh Man-Kyu
Seo Hee-Won
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Abstract
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Genus Sorbus is a long lived woody species. Plants of this genus are primarily distributed patchy throughout Asia and Europe. Sorbus commixta is primarily distributed throughout Europe. Eastern Asian Sorbus species are regarded as very important herbal medicines in Korea and China. Random amplified polymorphic DNA (RAPD) was used to investigate the genetic variation and phylogenetic analysis of four species of this genus in Korea. Although some Korean populations of these species were isolated and patchily distributed, they exhibited a high level of genetic diversity. Twenty-six primers revealed 205 loci, of which 128 were polymorphic (62.4%). S. commixta showed the highest diversity (0.165), whereas S. aucuparia showed the lowest diversity (0.109). The estimated gene flow (Nm) was low high among intra-species (mean Nm = 0.755). A similarity matrix based on the proportion of shared fragments (GS) was used to evaluate relatedness among species. The estimate of GS ranged from 0.786 to 0.963. The molecular data allowed us to resolve well-supported clades in Korean taxa and European species. An addition, especially, species-specific markers for genus Sorbus by RAPD analysis may be useful in germ-plasm classification and agricultural process of several taxa of this genus.
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KEYWORD
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Sorbus, RAPD, genetic variation, phylogenetic analysis
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